These are the Billboard magazine R&B singles chart number one hits of 1966:
See also: 1966 in music, List of number-one R&B hits (United States)
| Issue Date | Song | Artist |
| January 1 | I Got You (I Feel Good) | James Brown |
| January 8 | A Sweet Woman Like You | Joe Tex |
| January 15 | I Got You (I Feel Good) | James Brown |
| January 22 | Uptight (Everything's Alright) | Stevie Wonder |
| January 29 | Uptight (Everything's Alright) | Stevie Wonder |
| February 5 | Uptight (Everything's Alright) | Stevie Wonder |
| February 12 | Uptight (Everything's Alright) | Stevie Wonder |
| February 19 | Uptight (Everything's Alright) | Stevie Wonder |
| February 26 | Baby Scratch My Back | Slim Harpo |
| March 5 | Baby Scratch My Back | Slim Harpo |
| March 12 | 634-5789 (Soulsville, U.S.A.) | Wilson Pickett |
| March 19 | 634-5789 (Soulsville U.S.A.) | Wilson Pickett |
| March 26 | 634-5789 (Soulsville U.S.A.) | Wilson Pickett |
| April 2 | 634-5789 (Soulsville U.S.A.) | Wilson Pickett |
| April 9 | 634-5789 (Soulsville U.S.A.) | Wilson Pickett |
| April 16 | 634-5789 (Soulsville U.S.A.) | Wilson Pickett |
| April 23 | 634-5789 (Soulsville U.S.A.) | Wilson Pickett |
| April 30 | Get Ready | The Temptations |
| May 7 | When a Man Loves a Woman | Percy Sledge |
| May 14 | When a Man Loves a Woman | Percy Sledge |
| May 21 | When a Man Loves a Woman | Percy Sledge |
| May 28 | When a Man Loves a Woman | Percy Sledge |
| June 4 | It's a Man's Man's Man's World | James Brown |
| June 11 | It's a Man's Man's Man's World | James Brown |
| June 18 | Hold On! I'm a Comin' | Sam & Dave |
| June 25 | Ain't Too Proud to Beg | The Temptations |
| July 2 | Ain't Too Proud to Beg | The Temptations |
| July 9 | Ain't Too Proud to Beg | The Temptations |
| July 16 | Ain't Too Proud to Beg | The Temptations |
| July 23 | Let's Go Get Stoned | Ray Charles |
| July 30 | Ain't Too Proud to Beg | The Temptations |
| August 6 | Ain't Too Proud to Beg | The Temptations |
| August 13 | Ain't Too Proud to Beg | The Temptations |
| August 20 | Ain't Too Proud to Beg | The Temptations |
| August 27 | Blowin' in the Wind | Stevie Wonder |
| September 3 | You Can't Hurry Love | The Supremes |
| September 10 | You Can't Hurry Love | The Supremes |
| September 17 | Land of a Thousand Dances | Wilson Pickett |
| September 24 | Beauty Is Only Skin Deep | The Temptations |
| October 1 | Beauty Is Only Skin Deep | The Temptations |
| October 8 | Beauty Is Only Skin Deep | The Temptations |
| October 15 | Beauty Is Only Skin Deep | The Temptations |
| October 22 | Beauty Is Only Skin Deep | The Temptations |
| October 29 | Reach Out I'll Be There | The Four Tops |
| November 5 | Reach Out I'll Be There | The Four Tops |
| November 12 | Love Is a Hurtin' Thing | Lou Rawls |
| November 19 | Knock On Wood | Eddie Floyd |
| November 26 | You Keep Me Hangin' On | The Supremes |
| December 3 | You Keep Me Hangin' On | The Supremes |
| December 10 | You Keep Me Hangin' On | The Supremes |
| December 17 | You Keep Me Hangin' On | The Supremes |
| December 24 | (I Know) I'm Losing You | The Temptations |
| December 31 | (I Know) I'm Losing You | The Temptations |
|
||||||||||||||